Volume 14, Number 1, March 2002

Specific Identification of Telomerase Activity and p53 Gene Product in Metachronous Multiple Oral Squamous Cell Carcinoma and Adjacent Tissues

Yuuichi Sasakura,1 Yoshiko Miyoshi,2 Keiichi Tsukinoki,2 Junichi Shindo,1 Yoshihisa Watanabe2
1First Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, Kanagawa, Japan
2Department of Oral Pathology, Kanagawa Dental College, Kanagawa, Japan


Abstract
Objectives: To define the association of telomerase activity, histopathological characteristics, and p53 gene product expression in metachronous oral squamous cell carcinomas.
Patients and Methods: Three regions of tissue specimens from the tumour, the region adjacent to the tumour, and intact oral mucosa from the surgical margin from 2 patients were simultaneously evaluated. Telomerase activity was determined by telomeric repeat amplification protocol assay. Hematoxylin and eosin stain and DO7 monoclonal antibody were used to stain formalin-fixed, paraffin-embedded sections to examine the histopathological features and p53 gene product expression.
Results: Marked telomerase activity was expressed in tumour tissue and in adjacent dyskeratotic tissue. No telomerase activity was found in hyperkeratotic or dysplastic oral mucosa. Although p53 gene product was detected in the tumour and adjacent regions of both patients, it was found in the intact oral mucosa of only 1 patient.
Conclusion: Estimation of tissue telomerase activity and p53 gene product expression is helpful in identifying malignancy in oral mucosal lesions.

Key words: Telomerase, Mouth neoplasms, Histopathology

Asian J Oral Maxillofac Surg. 2002;14:27-34.
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