Volume 14, Number 1, March 2002
| | Histamine Release from Rat Mast Cells Induced by Human α-Defensin-1 Present in Jaw Cyst Fluid |
|---|
Nobuyoshi Mizukawa, Koichi Sawaki, Masayuki Kamio, Joji Fukunaga, Takaaki Ueno, Shin Takagi, Toshio Sugahara
Department of Oral and Maxillofacial Surgery 1, Okayama University Dental School, Okayama, Japan
Abstract
Objective: To investigate the release of histamine from rat mast cells elicited by human α-defensin-1 contained in jaw cyst fluid.
Patients and Methods: Human α-defensin-1 was purified by reversed-phase high-performance liquid chromatography from human jaw cyst fluid, and the mast cells were collected from the peritoneal cavities of rats.
Results: Histamine release was induced in isolated rat mast cells by both extracted and synthetic human α-defensin-1. Changes in vascular permeability induced by human α-defensin-1 were also estimated by the passive skin test in rats. Extracted and synthetic human α-defensin-1 induced histamine release from isolated rat mast cells in a dose-dependent manner over a concentration range of 1 to 15 µg/mL; the histamine release induced by the extracted and synthetic human α-defensin-1 (15 µg/mL) was 39.4 ± 2.3 and 37.6 ± 1.8%, respectively. When human α-defensin-1 was injected into rat skin intradermally, the vascular permeability increased. This response occurred with as little as 0.1 µg of human α-defensin-1, and a strong response was observed with 0.5 and 1.0 µg. These responses were completely abolished upon injection with 1 µg of the antihistamine drug diphenhydramine hydrochloride.
Conclusion: Human α-defensin-1 in human jaw cyst fluid can act as an inducer of histamine release from mast cells both in vivo and in vitro.
Key words: Defensins, Mast cells, Histamine release, Jaw cysts, Cyst fluid
Asian J Oral Maxillofac Surg. 2002;14:35-39.
 |
|
|
|
