Volume 14, Number 2, June 2002

Human Papillomavirus in Oral Squamous Papilloma and Verrucous Carcinoma: Evaluation Using in Situ Polymerase Chain Reaction Detection

Koichi Mishima, Yoshiki Nariai, Seiji Obara, Yasuro Yoshimura
Department of Oral and Maxillofacial Surgery, Shimane Medical University School of Medicine, Shimane, Japan

Objective: Evaluation of human papillomavirus infection in oral squamous papillomas and verrucous carcinomas using in situ polymerase chain reaction.
Patients and Methods: Thirteen patients with oral squamous papilloma and 8 with verrucous carcinoma were evaluated.
Results: In situ polymerase chain reaction showed that basal layer cells were specifically stained. The total human papillomavirus positivities were 84.6% (11) for patients with papilloma and 75.0% (6) for patients with verrucous carcinoma. Among patients with papilloma, 53.8% (7) were positive for human papillomavirus-16, 61.5% (8) for human papillomavirus-18, 23.0% (3) for human papillomavirus-6, and 23.0% (3) for human papillomavirus-11. Among patients with verrucous carcinoma, 12.5% (1) were positive for human papillomavirus-16 and 37.5% (3) for human papillomavirus-18, but no patient showed clear positivity for human papillomavirus-6 or -11.
Conclusion: In situ polymerase chain reaction is as sensitive as polymerase chain reaction of the extracts and specifically identifies the affected cells in the lesions, resulting in a high level of detection of human papillomavirus infections in these lesions.

Key words: Carcinoma, verrucous, Papilloma, Papillomavirus, human, Polymerase chain reaction, Tumour

Asian J Oral Maxillofac Surg. 2002;14:95-99.
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