Volume 14, Number 2, June 2002
| | Human Papillomavirus in Oral Squamous Papilloma and Verrucous Carcinoma: Evaluation Using in Situ Polymerase Chain Reaction Detection |
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Koichi Mishima, Yoshiki Nariai, Seiji Obara, Yasuro Yoshimura
Department of Oral and Maxillofacial Surgery, Shimane Medical University School of Medicine, Shimane, Japan
Abstract
Objective: Evaluation of human papillomavirus infection in oral squamous papillomas and verrucous carcinomas using in situ polymerase chain reaction.
Patients and Methods: Thirteen patients with oral squamous papilloma and 8 with verrucous carcinoma were evaluated.
Results: In situ polymerase chain reaction showed that basal layer cells were specifically stained. The total human papillomavirus positivities were 84.6% (11) for patients with papilloma and 75.0% (6) for patients with verrucous carcinoma. Among patients with papilloma, 53.8% (7) were positive for human papillomavirus-16, 61.5% (8) for human papillomavirus-18, 23.0% (3) for human papillomavirus-6, and 23.0% (3) for human papillomavirus-11. Among patients with verrucous carcinoma, 12.5% (1) were positive for human papillomavirus-16 and 37.5% (3) for human papillomavirus-18, but no patient showed clear positivity for human papillomavirus-6 or -11.
Conclusion: In situ polymerase chain reaction is as sensitive as polymerase chain reaction of the extracts and specifically identifies the affected cells in the lesions, resulting in a high level of detection of human papillomavirus infections in these lesions.
Key words: Carcinoma, verrucous, Papilloma, Papillomavirus, human, Polymerase chain reaction, Tumour
Asian J Oral Maxillofac Surg. 2002;14:95-99.
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