Volume 16, Number 3, September 2004
| | Response of Osteoblastic Cells to Implant Materials: CD44 Can Transmit a Signal for Formation of Dendritic Processes on Osteoblastic Cells |
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Atsushi Danjo, Yoshio Yamashita, Akira Okumura, Masaaki Goto
Department of Oral and Maxillofacial Surgery, Saga Medical School, Saga, Japan
Abstract
Objective: To evaluate the difference between the effects of hydroxyapatite and titanium implant materials on the morphology of human osteoblastic cells.
Patients and Methods: The human osteoblastic cells Saos-2 were cultured onto hydroxyapatite and titanium disks. The cultured cells were compared for the expression pattern of cell surface adhesion molecules by flow cytometry. Saos-2 cells were plated on monoclonal antibody-coated wells for spreading assay.
Results: Saos-2 cells grown on hydroxyapatite developed numerous long thin filopodia, similar to dendritic cells. The cells on titanium showed spreading and lamelipodia formation. The cultured cells on hydroxyapatite had a lower intensity of CD44 than those on titanium. Moreover, monoclonal antibody against human CD44 induced a rapid and extensive formation of dendrite processes in Saos-2 cells.
Conclusion: The signalling through CD44 on the cell surface might be an important factor controlling the response of spreading osteoblasts.
Key words: CD44, Cell adhesion molecules, Flow cytometry, Implant materials, Osteoblast
Asian J Oral Maxillofac Surg. 2004;16:177-183.
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