Volume 17, Number 1, March 2005
| | Immunosuppressive Cytokine Interleukin-10 mRNA Expression Correlates with Tumour Progression in Oral Squamous Cell Carcinoma |
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Kenji Suzuki,1 Eiro Kubota,1 Satoshi Shimizu,1 Shigeyuki Ozawa,1 Hideo Imamura,2 Masaaki Goto,2 Takeshi Katsuki2
1Department of Oral and Maxillofacial Surgery, Kanagawa Dental College, Kanagawa, and 2Department of Oral and Maxillofacial Surgery, School of Medicine, Saga University, Saga, Japan
Abstract
Objective: To examine the correlation between immunosuppressive cytokine interleukin-10 mRNA expression and clinicopathological characteristics of patients with oral squamous cell carcinoma.
Patients and Methods: Expression of interleukin-10 mRNA in tissues taken from 34 patients with oral squamous cell carcinoma was examined by reverse transcription-polymerase chain reaction and immunohistochemical analysis.
Results: The cDNA encoding for T-lymphocyte receptor complex, CD3-δ, was amplified in all samples, indicating the presence of tumour infiltrating lymphocytes. Interleukin-10 mRNA was detected in 21 of 34 samples (61.8%). Densitometric analysis of the cDNA bands demonstrated that the ratio of CD3-δ to control β-actin was significantly lower in patients with advanced-stage carcinoma compared with those with early-stage disease (p = 0.047). However, the ratio of interleukin-10 to CD3-δ was significantly higher in the advanced stages than in the early stages (p = 0.012).
Conclusions: These results suggest that tumour infiltrating lymphocytes decrease with progression of the tumour, whereas interleukin-10 expression remains constant around the tumour. These data also suggest that interleukin-10 may be produced not only by T cells but also by tumour cells.
Key words: Carcinoma, squamous cell, Interleukin-10, Reverse transcriptase polymerase chain reaction
Asian J Oral Maxillofac Surg. 2005;17:11-19.
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