Volume 17, Number 1, March 2005
| | Photodynamic Therapy for Squamous Cell Carcinoma Invading Alveolar Bone in a Mouse Model |
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Akihiko Irie, Tsugio Inokuchi, Joji Sekine, Hisazumi Ikeda
Division of Oral and Maxillofacial Surgical Reconstruction and Functional Restoration, Department of Developmental and Reconstructive Medicine, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, Japan
Abstract
Objective: To examine the effects of photodynamic therapy on squamous cell carcinoma invading the alveolar bone.
Materials and Methods: NR-S1 tumour was transplanted into an upper incisor extraction socket of 5-week-old male C3H/HeNCrj mice. Twenty six mice were divided into 4 groups: 8 received no treatment, 5 received a photosensitiser (haematoporphyrin oligomers), 5 underwent neodymium:YAG laser irradiation, and 8 received a photosensitiser followed by laser irradiation. The area of tumour necrosis was measured by image analysis of haematoxylin and eosin-stained specimens. Proliferative activity of cells in the tumour and normal tissue was evaluated by immunohistochemistry.
Results: The patterns of tumour growth and spread were similar in the control, photosensitiser, and laser groups. Although photosensitiser followed by laser irradiation induced tumour necrosis at a higher rate than the other 3 treatments, surviving tumour cells were found at the tumour margin and in the extraction socket.
Conclusion: Photosensitiser followed by laser irradiation is not an effective treatment for squamous cell carcinoma involving alveolar bone.
Key words: Alveolar bone loss, Bromodeoxyuridine, Carcinoma, squamous cell, Hematoporphyrin photoradiation, Immunohistochemistry
Asian J Oral Maxillofac Surg. 2005;17:20-25.
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