Volume 19, Number 1, March 2007
| | Feasibility of Blue Dye Method to Identify Sentinel Lymph Node Metastasis in Patients with Oral Cancer |
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Hiroyuki Yamada,1 Koji Kawaguchi,1 Susumu Iketani,2 Kanichi Seto1
1First Department of Oral and Maxillofacial Surgery, Tsurumi University School of Dental Medicine, Yokohama, and 2Department of Oral and Maxillofacial Surgery, Toshiba Rinkan Hospital, Yokohama, Japan
Abstract
Objective: To examine the feasibility of a combination of the blue dye injection method and reverse transcriptase-nested polymerase chain reaction for squamous cell carcinoma antigen mRNA to detect sentinel lymph node metastasis in patients with oral cancer.
Patients and Methods: Ten individuals with previously untreated oral cancer participated in the study. Sentinel lymph nodes were identified by the blue dye injection method during neck dissection. We investigated the comparative effectiveness of frozen haematoxylin and eosin section analysis and molecular analysis of squamous cell carcinoma antigen for sentinel lymph node diagnosis.
Results: In 8 out of the 10 cases, sentinel lymph nodes were identified. One histologically positive sentinel lymph node also showed positive for squamous cell carcinoma antigen. The remaining 7 histologically negative sentinel lymph nodes were also negative for squamous cell carcinoma antigen. However, in 3 cases, squamous cell carcinoma antigen-positive non-sentinel lymph nodes were detected despite sentinel lymph nodes being negative for squamous cell carcinoma antigen.
Conclusion: Our preliminary results suggest that the combination of the single blue dye injection method and reverse transcriptase-nested polymerase chain reaction for the squamous cell carcinoma antigen mRNA was not very accurate for detection of sentinel lymph node metastasis in patients with oral cancer.
Key words: Coloring agents, Mouth neoplasms, Predictive value of tests, Reverse transcriptase polymerase chain reaction, Sentinel lymph node biopsy, Squamous cell carcinoma
Asian J Oral Maxillofac Surg. 2007;19:19-24.
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