Volume 19, Number 2, June 2007
| | Apolipoprotein E Stimulates Bone Formation on Titanium in Vitro |
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Takashi Kodama,1 Tetsuya Goto,2 Toru Ishibe,1 Shigeru Kobayashi,2 Tetsu Takahashi1
1Division of Oral and Maxillofacial Reconstructive Surgery, and 2Division of Anatomy, Kyushu Dental College, Kitakyushu, Japan
Abstract
Objective: To examine the effects of apolipoprotein E2, a strongly expressed molecule during titanium implant healing, on bone formation by rat primary osteoblasts over titanium.
Materials and Methods: Primary rat osteoblasts were cultured on titanium in the presence of 0.01-1.0 μg/mL apolipoprotein E2. After 2 or 3 weeks of culture, bone formation was analysed by staining the bone-like nodules with calcein blue. The mRNAs of the osteogenic genes type I collagen, osteopontin, and osteocalcin, were analysed using semi-quantitative reverse transcriptase-polymerase chain reaction.
Results: Apolipoprotein E2 enhanced bone-like nodule formation on titanium in a dose-dependent manner. Apolipoprotein E2 strongly upregulated osteopontin and osteocalcin mRNA expression, but had only a small effect on type I collagen mRNA expression.
Conclusion: Apolipoprotein E2 stimulated bone formation on titanium disks in vitro. This may lead to a new approach to accelerating implant healing.
Key words: Apolipoproteins, Bone and bones, Osteoblasts, Osteocalcin, Titanium
Asian J Oral Maxillofac Surg. 2007;19:96-100.
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