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Volume 19, Number 2, June 2007

Apolipoprotein E Stimulates Bone Formation on Titanium in Vitro

Takashi Kodama,1 Tetsuya Goto,2 Toru Ishibe,1 Shigeru Kobayashi,2 Tetsu Takahashi1
1Division of Oral and Maxillofacial Reconstructive Surgery, and 2Division of Anatomy, Kyushu Dental College, Kitakyushu, Japan

Abstract
Objective: To examine the effects of apolipoprotein E2, a strongly expressed molecule during titanium implant healing, on bone formation by rat primary osteoblasts over titanium.
Materials and Methods: Primary rat osteoblasts were cultured on titanium in the presence of 0.01-1.0 μg/mL apolipoprotein E2. After 2 or 3 weeks of culture, bone formation was analysed by staining the bone-like nodules with calcein blue. The mRNAs of the osteogenic genes type I collagen, osteopontin, and osteocalcin, were analysed using semi-quantitative reverse transcriptase-polymerase chain reaction.
Results: Apolipoprotein E2 enhanced bone-like nodule formation on titanium in a dose-dependent manner. Apolipoprotein E2 strongly upregulated osteopontin and osteocalcin mRNA expression, but had only a small effect on type I collagen mRNA expression.
Conclusion: Apolipoprotein E2 stimulated bone formation on titanium disks in vitro. This may lead to a new approach to accelerating implant healing.


Key words: Apolipoproteins, Bone and bones, Osteoblasts, Osteocalcin, Titanium

Asian J Oral Maxillofac Surg. 2007;19:96-100.
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